Lab #4: The Biomolecule of Vampirism
I searched for draculin, a protein found in the saliva of Desmodus rotundus, otherwise known as the common vampire bat. The specific protein I chose to examine was Draculin-1, partial [Desmodus rotundus]. The accession number for this protein is JAA65087.1. The amino acid sequence for this protein is:
"1 mkllflalls llalgpslaa rrrgvrwcti skpeaakcsk lqqnlkrvrg pslscisrks
61 yleciqaiaa kradamslda glvyeagqdp yrlrpvaaev ygtegaprth yyavalvkkd
121 snlqlnqlqg vrschtglnr sagwkipvgt lrpylgwagp paplqeavan ffsascvpca
181 dgnqypnlcr lcagtgadkc acsskepyfg ysgafkclkd gagdvafvkd stvfenlpnk
241 aerdqyellc pdntrkpvde feqchlarvp shavvarsvg gkedsiwrll skaqekfgkg
301 tsgsfqlfss ppgqkdllfk dgaqgflrip srvdaelylg psyltviknl ngigsrggdp
361 ggpglcgarw aqrscasass gvaramgq,"
with the first 10 amino acids being methionine, lysine, leucine, leucine, phenylalanine, leucine, alanine, leucine, leucine, and serine.
The nucleotide sequence which codes for this form of draculin is: "1 atgaagctcc tcttccttgc actgctgtcc ctcctggccc tcgggccgag tctggctgcc 61 cgcaggagag gtgttcgatg gtgcaccata tcaaaaccag aggcagcaaa atgctctaaa 121 ctgcaacaga atctaaaacg agtgcgtggc ccctctctct cctgcataag cagaaagtcc 181 tacctggaat gtatccaggc catcgcggcg aaaagggcag atgccatgag ccttgatgca 241 ggtctggtgt acgaggcagg acaggacccg tacagactgc ggcctgtggc agcagaggtc 301 tacgggaccg agggggcacc gcggacgcac tattacgctg tggccctggt gaaaaaggac 361 agcaacttgc aactgaacca gctgcaaggc gtgaggtcct gccacactgg cctcaacagg 421 tccgccgggt ggaaaatccc tgtgggcacg ctccgtccgt acctgggctg ggcagggcca 481 cctgcacccc tccaggaagc tgtggccaac ttcttctccg ctagctgtgt tccctgtgca 541 gatggcaacc agtaccccaa cctgtgtcgc ttgtgtgcgg ggacaggggc agataaatgt 601 gcctgctcct ccaaggaacc gtactttggc tactccggtg ccttcaagtg tctgaaagat 661 ggggctggag acgtggcttt tgtcaaggac agtacggtgt ttgagaacct gccaaacaag 721 gccgagagag accagtatga gctgctctgc ccagacaaca cccgaaagcc ggtggatgag 781 tttgagcagt gccacctggc ccgggtccct tctcatgcag ttgtggcccg aagcgtgggt 841 ggcaaggagg actcgatctg gaggcttctc agcaaggcac aggagaagtt tggaaaaggc."
Reference:
B.G. Fry. "Molecular phylogeny and evolution of the proteins encoded by the common vampire bat (Desmodus rotundus) submaxillary glands." Unpublished.

Hi Caleb,
ReplyDeleteYour protein choice is definitely interesting! Just the name alone made me wonder if that name derived from the fictional character, or vice versa. I have heard that draculin is used in the medical field since it can help with blood clots, but I do not know if it is being used legally or not.
Wow, a thrilling pic. A quick search on Wikipedia explained how this protein works as an anticoagulant in order to keep the blood of the victim flowing. Just a couple days I saw a video on penguins and how this mammal is a common threat to them. I wonder what the phylogeny tree for these bats look like and if once they obtained this draculin protein if that part of the clade radiated from there.
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