Lab #4: BLAST by Hannah Zaini

Lab#4: BLAST by Hannah Zaini

For this assignment, I searched for the protein metalloprotease, which is an enzyme in snake venom. This enzyme inhibits and suppresses human platelet adhesion to collagen. Essentially, it is a part of the venom that kills people. I chose it because I thought it would be really cool to look at the components of snake venom. 

On the website, I selected metalloprotease in the organism Gloydius Halys, which is the scientific name for the Halys Viper. 

Here is a picture of the Halys Viper

Here are the 11 lines of the amino acid sequence for metalloprotease: 

 1 miqvllvtic lavfpyqgss iilesgnvnd yevvyprkvp alpkgavqpk yedamqyefk
      61 vngepvvlhl eknkglfsed ysethyspdg reittnppve dhcyyhgriq ndadstasis
     121 acnglkghft lqgetyliep lklpdseaha vfkyenveke deapkmcgvt qnwesyepik
     181 kasqsnltpe qqrylnakky vklvmvadyi mylkydrnlt tvrtrmydiv nvinviyqrm
     241 nihvalvgle iwsnkdkfil rsaadvtlkl fatwretdll krkshdnaql ltginfngpt
     301 aglgylggic npmysagivq dhnkihhlva iamahemghn lgidhdkdtc tcgakscvma
     361 gtlsceasyl fsdcsrkehq aflikdmpqc ilkkplktdv vsppvcgnyf vevgedcdcg
     421 spatcrdscc daatcklrqg aqcaeglccd qcrfkgagte craatdecdm adlctgrsae
     481 ctdrfqrngq pcqnnngycy ngkcpimtdq cialfgpnaa vsedacfqfn legnhygycr
     541 keqntkiace pqnvkcgrly cidsspankn pcniyyspgd edkgmvlpgt kcadgkacsn
     601 gqcvdvnras

Here are the first 10 amino acids translated:

Methionine Isoleucine Glutamine Valine Leucine Leucine Valine Threonine Isoleucine Cysteine 

This is the citation: 

You, Weon-Kyoo, et al. “A Novel Disintegrin-like Domain of a High Molecular Weight Metalloprotease Inhibits Platelet Aggregation.” Biochemical and Biophysical Research Communications, vol. 309, no. 3, 2003, pp. 637–642., doi:10.1016/j.bbrc.2003.08.049.

Here are the first 15 lines of the mRNA nucleotide sequence that codes for my protein:

 1 ccagcctcca aaatgatcca agttctcttg gtaactatat gcttagcagt ttttccttat
      61 caagggagtt ctataatcct ggaatctggg aacgtgaatg attatgaagt agtgtatcca
     121 cgaaaagtcc ctgcattgcc caaaggagca gttcagccaa agtatgaaga cgccatgcaa
     181 tatgaattta aagtgaatgg agagccagtg gtccttcacc tggaaaaaaa taaaggactt
     241 ttttcagaag attacagcga gactcattat tcccctgatg gcagagaaat tacaacaaac
     301 cctccagttg aggatcactg ctattatcat ggacgcatcc agaatgatgc tgactcaact
     361 gcaagcatca gtgcatgcaa cggtttgaaa ggacatttca cgcttcaagg ggagacatac

421 cttattgaac ccttgaaact tcccgacagt gaagcccatg cagtcttcaa atatgaaaac
     481 gtagaaaaag aggatgaggc ccccaaaatg tgtggggtaa cccagaattg ggaatcatat
     541 gagcccatca aaaaggcctc tcagtcaaat cttactcctg aacaacaaag atacttgaac
     601 gccaaaaaat acgtgaagct tgtcatggtt gcagactaca taatgtactt gaaatatgac
     661 cgcaatttaa ctactgtaag aacaagaatg tatgatattg tcaacgttat aaatgtgatt
     721 taccaacgta tgaatattca tgtagcactg gttggcctag aaatttggtc caacaaagat
     781 aaatttatcc tgcggtcagc agcggatgtt actttgaagt tatttgcaac ctggagagag
     841 acagatttgc tgaagcgcaa aagtcacgat aatgcccagt tactcacggg cattaatttc