Lab #4: Chlorea Toxin (GenBank: BAA06291.1) - Samuel Robinson

For this lab I chose to look at the protein Cholera Toxin. Cholera Toxin is primarily found in the bacteria Vibrio cholerae. I chose this protein because I have heard mention of it several times, but never really new what it did.

The amino acid sequence for Cholera toxin (GenBank: BAA06291.1):

        1 miklkfgvff tvllssayah gtpqnitdlc aeyhntqiyt lndkifsyte slagkremai
       61 itfkngaifq vevpgsqhid sqkkaiermk dtlriaylte akveklcvwn nktphaiaai
      121 sman

Reference data is drawn from: 

Yamamoto, K. et al. "Comparison of cholera toxin genes (ctxAB) of non-O1 vibrio cholerae strains 854 (O139-bengal) and S7 (O37) from two outbreaks"

Translation for First 10 Amino Acids:

Methionine, Isoleucine, Lysine, Leucine, Lysine, Phenylalanine, Glycine, Valine, Phenylalnine, Phentlalanine.

Nucleotide Sequence (first 15 lines)

        1 actaaatagt atattttgat ttttgatttt tgatttcaaa taatacaaat ttatttactt
       61 atttaattgt tttgatcaat tatttttctg ttaaacaaag ggagcatata tggtaaagat
      121 aatatttgtg ttttttattt tcttatcatc attttcatat gcaaatgatg ataagttata
      181 tcgggcagat tctagacctc ctgatgaaat aaagcagtca ggtggtctta tgccaagagg
      241 acagagtgag tactttgacc gaggtactca aatgaatatc aacctttatg atcatgcaag
      301 aggaactcag acgggatttg ttaggcacga tgatggatat gtttccacct caattagttt
      361 gagaagtgcc cacttagtgg gtcaaactat attgtctggt cattctactt attatatata
      421 tgttatagcc actgcaccca acatgtttaa cgttaatgat gtattagggg catacagtcc
      481 tcatccagat gaacaagaag tttctgcttt aggtgggatt ccatactccc aaatatatgg
      541 atggtatcga gttcattttg gggtgcttga tgaacaatta catcgtaata ggggctacag
      601 agatagatat tacagtaact tagatattgc tccagcagca gatggttatg gattggcagg
      661 tttccctccg gagcatagag cttggaggga agagccgtgg attcatcatg caccgccggg
      721 ttgtgggaat gctccaagat catcgatgag taatacttgc gatgaaaaaa cccaaagtct
      781 aggtgtaaaa ttccttgacg aataccaatc taaagttaaa agacaaatat tttcaggcta
      841 tcaatctgat attgatacac ataatagaat taaggatgaa ttatgattaa attaaaattt