BLAST-AB By David Rowlett

1. For this assignment I thought it would be cool to look at a very dangerous protein. I searched on google the most dangerous proteins in the world and found on the website https://io9.gizmodo.com/5915470/10-of-the-deadliest-proteins-on-earth that botulinum toxin was the most dangerous, only requiring 100 nano grams to kill the average human. However, many people have heard of this protein because it is also used in Botox injections to paralyze the facial muscles to eliminate wrinkles. 
2. The specific protein I chose was botulinum toxin found in the bacteria Erwinia amylovora. The accession number is ATZ10321
3. The amino acid sequence that codes for this protein:
1 mdmynwdmvc avscrdlnke lkksvrnnfg efswadgagn risgmfdrwe ivpggdvqrl

61 nlitplitgk leasvlgkdm nvavdnlcpk lqvvlafvsg engddthlkf ncrristsst 121 etasegsvvi lendinnpla drdnivpalf calmakmiva rrndlkfifa eiltipatse 181 vswmklhllr yaynekisge lgclavlgil rsnafpphpd elqpvfdssl vrddgnsgfm 241 lsrqvfmknv vlpalptvfk gaeisqfyla dgnvirnsgn islspingyt pyfnhfemev 301 vdlrimlnna rgrcdvvrns syvsfdlsaa yipqlsvvng vckvnlvcat rpvfnsqgyd 361 taaqifwifg gwvvdaliqg irsqmehllf efgnrgisfd iypikfssas eytecglaen 421 ffmrnThe names of the first 10 amino acids are: Methionine (start), Aspartic Acid, Methionine, Tyrosine,

Asparagine, Tryptophan, Aspartic Acid, Methionine, Valine, and Cysteine.

Figure 1: Representation of Botulinum Toxin protein structure from io9.gizmodo.com

4. Reference:

NCBI:

Authors: Lagonenko,A.L., Komardina,V.S., Nikolaichik,Y.A. and

Evtushenkov,A.N.

Title: First Report of Erwinia amylovora Fire Blight in Belarus
Journal: J Phytopathol (1986) 156 (10), 638-640 (2008)

Veronese K. 10 of the Deadliest Proteins on Earth. io9. 2012 Jun 19 [accessed 2018 Jan 17]. https://io9.gizmodo.com/5915470/10-of-the-deadliest-proteins-on-earth

5. Nucleotide Sequence:

1 aaaggcgatc gctgcccgat cgcctccctg ttcagatcaa tgtcttccag gtttccagcc

       61 attcgccggc gggatcttcc gggatttcat gttcaagtac gtcaatttcc agacgctcgc
      121 cgatacgctg cgcacccaga gaggtcagca gctggtcaaa ttgtctgacg gcgccgcaga
      181 acagatcgta ttcgcggtta ccgaggccaa cagcgccgta gcgcaactga ctcaggtcgg
      241 gctgctgttc cttaatcgca tcgaacagcg gctgaatgtt gtcgggtaat tcaccggccc
      301 cgtgggtgga ggtcacgata atccagatcc cttccagctt cagttctgcc agctccggcc
      361 cgtggagcgt ggtggtgttg tgaccggctt cagaaagctg ctcatccaga tgatcggcca
      421 cgtattccga gctgcccagc gtactgccac tgattaaagt aatgtcggcc ataatctgat
      481 cccagtgtga taatggcggg cattgtacgc tgtgatcagg ctgggatcta cctgtgggta
      541 agtagggata tttcttaatt tcattggctt tgctggccat cgtgatcata gccatgttta
      601 tcgccaggct gtctgtaaac ggattaagca gaacctggcg cagtcgcaac gcggtgcccg
      661 acttacagaa acgggagggg ttggctgtgg ataatttcat gcaccgatca tgggataatc
      721 gtacgcatga tcgggttttg caacgagatc agggtttcag ttgactggat ctcatcgatg
      781 gtctggatct tgttaatcag tacctgttgc agggcgtcga tcgaacggca catcacttta
      841 ataaagatgc tgtaatgtcc ggtggtgtac caggcttcca ccacttcttc aagctgctcc