Blog Post #2 Lydia Mikhail

The protein I searched for is polyphenol oxidase, which is an enzyme found in a lot of fruit and vegetables as well as coffee and tea. I chose this protein because on a whim and realized that coffee beans have proteins in them too! Proteins really are the answer to everything!

The amino acid sequence for the polyphenol oxidase precursor is:

        1 maslqslfip tittrttlpn ptsnrrskty pkvscnatsd dnekllilpe tqklilpttl

       61 nvdrrnlllg lgaglystvn ftsapaafad pittpdistc vastkgfqpk navrtnaccp

      121 ptlsktvkef vfpndratri rpaahraspe yiakykaaiq kmrdledddp hsfvsqakih

      181 caycnggftq dkhpdkelqi hnswlffpfh rwylyfyeri lgkliddptf aipywnwdnp

      241 agmslpdffe eggkanpafd ayrnsshlap aiadldydgt eknytcikql svnlgamnkq

      301 mirnagdwks ffggeyrags dpignkdpsv gsveagchta ihrwvgnsrl pngedmgnfy

      361 sagydpvfyv hhanvdrmwk vwkdlgirgh ieppdndwrn asyvfydenk elvrvynkdc

      421 vrleklkydy qfapipwkns rpvartkksn ialksvgtvk qaqdskfplk ldkttkvlvk

      481 rpatnrsked kenanevlli kgvvfngekf vkfdvfvndk ddvtastaee sefagsfaql

      541 phnhgdkmlm tssarfglte lledidaedd esilvtlvpk vgceevtige ikvelvpiee

The first 10 amino acids translate to:

Methionine-alanine-serine-leucine-glutamine-serine-leucine-phenylalanine-isoleucine-proline

The first 15 lines of the nucleotide sequence is:

        1 atggaatcct ttgcaccaac caacaacggc accggcatct cctccacggg tggaaggagc

       61 ttctactcat cctcatcgac ctactctgct tccttctctt ttacgtcatc tcaagttccc

      121 atcgccagaa ttatctccac cccccgccat gcaatttcat gcaaaaccca agatgatcat

      181 gaccaccgcc atgcaaactc cggcaaaatt gataggagaa acgtcctatt aggcctcgga

      241 actctctctg gtgccaccgc cacttttggg cctaattcac tagcttttgc tgatccgatc

      301 atggcacccg acatcacatt atgtggtccg gccaccttcc ccgaaggtgt tgccaccaca

      361 aactgctgcc ccccatccac cacaaagatt ctcgatttca aagttccatc acaggcaaac

      421 accctccgtg tccggccggc agcacatttg gttaataaag attacatagc caaatacaat

      481 aaagttgtcc agctaatgaa agctctccct gaagacgatc ctcgtaattt tatgcaacaa

      541 gctagtgttc attgtgcata ttgcgatggc acatatgacc aactcggttt ccaagatctc

      601 gagtttcaag tccatgcttc atggcttttc tatccttttc atcgctatta cctatacttc

      661 ttcgagaaaa tttgtggcaa attaatcgat gacccaaatt tcacaattcc attttggaac

      721 tgggacgcgc ctgatgggat gacaatccct tacatttaca caaataagaa ctcttcgtta

      781 tatgatagtc ttcgtgacgc aaagcatcaa ccaccatctt tggttgatct tgactacaat

      841 ggtgttgaca gaaatcttag cccttccaaa caaacgtcca caaatctcaa tattatgtat

Sources:

REFERENCE   1

  AUTHORS   Richter,C., Dirks,M.E., Gronover,C.S., Prufer,D. and

            Moerschbacher,B.M.

  TITLE     Silencing and heterologous expression of ppo-2 indicate a specific

            function of a single polyphenol oxidase isoform in resistance of

            dandelion (Taraxacum officinale) against Pseudomonas syringae pv.

            tomato

  JOURNAL   Mol. Plant Microbe Interact. 25 (2), 200-210 (2012)

REFERENCE   1

  AUTHORS   Dirks-Hofmeister,M.E., Inlow,J.K. and Moerschbacher,B.M.

  TITLE     Site-directed mutagenesis of a tetrameric dandelion polyphenol

            oxidase (PPO-6) reveals the site of subunit interaction

  JOURNAL   Plant Mol. Biol. 80 (2), 203-217 (2012)