Lab #4 Blast by Darsi Fouillade
1. The protein I searched for was IgG. This is a type of antibody or immunoglobulin. The IgG antibody is the only one of these (a group of them are shown below) that crosses the placenta during pregnancy. We touched upon this some in lab with our conversation about passive immunity and how a good thing can not be so good with Rh type factors involved. This structure contains disulfide bonds that connect the two chains together (one heavy and the other light.) The antigen bonding sites are at the top two ends of the protein.
2. The specific protein selected was for a western European house mouse: IgG, partial [Mus musculus domesticus]. Oh soo cute. The "partial" is in reference to the V-D-J region.
3. The amino acid sequence is:
1 evklvesgag vvkpggslkl sceasgfsfs ryvmswvrqt pekrlewvas issggrtyyp
61 gsemgrftis rdsarnilyl qmsslksedt amfycaredy yggrywyfdv wgagttvtvs
121 sa
Using the chart I found on the internet, I converted the first 10 amino acid 1-letter symbols to the corresponding 3-letter symbols, ie e is GLU, v is valine, k is lysine and so forth to come up with this sequence: Glu - Val - Lys - Leu - Val - Glu - Ser - Gly - Ala. The full names included would be: glutamic acid - valine - lysine - leucine - valine - glutamic acid - serine - glycine - alanine for the first 10 amino acids.
1 evklvesgag vvkpggslkl sceasgfsfs ryvmswvrqt pekrlewvas issggrtyyp
61 gsemgrftis rdsarnilyl qmsslksedt amfycaredy yggrywyfdv wgagttvtvs
121 sa
Using the chart I found on the internet, I converted the first 10 amino acid 1-letter symbols to the corresponding 3-letter symbols, ie e is GLU, v is valine, k is lysine and so forth to come up with this sequence: Glu - Val - Lys - Leu - Val - Glu - Ser - Gly - Ala. The full names included would be: glutamic acid - valine - lysine - leucine - valine - glutamic acid - serine - glycine - alanine for the first 10 amino acids.
4. Citation: Sawada,J., Yamazaki,T. and Terao,T., TITLE Molecular and biochemical analyses of combining sites of monoclonal anti-morphine antibodies, JOURNAL Mol. Immunol. 30 (1), 77-86 (1993)
5. The nucleotide sequence that codes
for this protein is:
1 gaagtgaagc tggtggagtc tggggcaggc gtagtgaagc ctggagggtc cctgaaactc
61 tcctgtgaag cctctggatt cagtttcagt agatatgtca tgtcttgggt tcgccagact
121 ccagagaaga gactggagtg ggtcgcatcc attagtagtg gtggtcgcac ctactatcca
181 ggcagtgaga tgggccgatt caccatttcc agagatagtg cgaggaacat cctatacctg
241 caaatgagca gtctgaagtc tgaagacacg gccatgtttt actgtgcnag agaggattac
301 tacggtggta gatattggta cttcgatgtc tggggcgctg ggaccacggt caccgtctcc
361 tcagccnaaa
61 tcctgtgaag cctctggatt cagtttcagt agatatgtca tgtcttgggt tcgccagact
121 ccagagaaga gactggagtg ggtcgcatcc attagtagtg gtggtcgcac ctactatcca
181 ggcagtgaga tgggccgatt caccatttcc agagatagtg cgaggaacat cctatacctg
241 caaatgagca gtctgaagtc tgaagacacg gccatgtttt actgtgcnag agaggattac
301 tacggtggta gatattggta cttcgatgtc tggggcgctg ggaccacggt caccgtctcc
361 tcagccnaaa
Hi Darci,
ReplyDeleteThe protein you chose was interesting to read about. I didn't know that IgG crossed the placenta during pregnancy. I researched more about it and found that it is found in all body fluids and protects against bacterial and viral infections. I also like that you posted an image of the different antibody classification; it gives us more detail.