Lab #4 : BLAST by Hart Kitsondhi
The protein that I have chosen for this blog assignment is Prion Protein or known as PrP and the one that I am looking at specifically is the PrP 129M. This protein is commonly found across species but the PrP 129M is located within Homo Sapiens. There is no significant function or anything special to this protein in terms of function whatsoever but in the situation where it is absence in the body, It could be deadly to the organism. If the PrP 129M is not present in the in the body, especially in the brain, it can causes an increase in oxidative stress, which is a lost in balance between "the production of free radicals and the ability of the body to counteract harmful affects". It makes this protein so redundant in terms of it being there.
The amino acids that codes for the protein are:
1 agcagtcatt atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag 61 tgacctgggc ctctgcaaga agcgcccgaa gcctggagga tggaacactg ggggcagccg 121 atacccgggg cagggcagcc ctggaggcaa ccgctaccca cctcagggcg gtggtggctg 181 ggggcagcct catggtggtg gctgggggca gcctcatggt ggtggctggg ggcagcccca 241 tggtggtggc tggggacagc ctcatggtgg tggctggggt caaggaggtg gcacccacag 301 tcagtggaac aagccgagta agccaaaaac caacatgaag cacatggctg gtgctgcagc 361 agctggggca gtggtggggg gccttggcgg ctacatgctg ggaagtgcca tgagcaggcc 421 catcatacat ttcggcagtg actatgagga ccgttactat cgtgaaaaca tgcaccgtta
The first 10 amino acids are: Serine, Serine, Histidine, Tyrosine, Glycine, Glutamine, Proline, Tryptophan, Leucine, Leucine.
Citation:
Kikuchi,Y., Kakeya,T., Nakajima,O., Sakai,A., Ikeda,K., Yamaguchi,N., Yamazaki,T., Tanamoto,K., Matsuda,H., Sawada,J. and Takatori,K.Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein, FEBS J. (2008) In press.The first 15 lines that codes for PrP 129M:1 agcagtcatt atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag 61 tgacctgggc ctctgcaaga agcgcccgaa gcctggagga tggaacactg ggggcagccg 121 atacccgggg cagggcagcc ctggaggcaa ccgctaccca cctcagggcg gtggtggctg 181 ggggcagcct catggtggtg gctgggggca gcctcatggt ggtggctggg ggcagcccca 241 tggtggtggc tggggacagc ctcatggtgg tggctggggt caaggaggtg gcacccacag 301 tcagtggaac aagccgagta agccaaaaac caacatgaag cacatggctg gtgctgcagc 361 agctggggca gtggtggggg gccttggcgg ctacatgctg ggaagtgcca tgagcaggcc 421 catcatacat ttcggcagtg actatgagga ccgttactat cgtgaaaaca tgcaccgtta 481 ccccaaccaa gtgtactaca ggcccatgga tgagtacagc aaccagaaca actttgtgca 541 cgactgcgtc aatatcacaa tcaagcagca cacggtcacc acaaccacca agggggagaa 601 cttcaccgag accgacgtta agatgatgga gcgcgtggtt gagcagatgt gtatcaccca 661 gtacgagagg gaatctcagg cctattacca gagaggatcg agcatggtcc tcttctcctc 721 tccacctgtg atcctcctga tctctttcct catcttcctg atagtgggat gaggaaggtc 781 ttcctgtttt caccatcttt ctaatctttt tccagcttga gggaggcggt atccacctgc 841 agccctttta gtggtggtgt ctcactcttt cttctctctt tgtcccggat aggctaatca
Hi Hart,
ReplyDeleteThe protein you have researched stands out to me just because it seems like the main function of the protein is to maintain chemical equilibrium with in the body. It makes me think if this protein is not create enough in the body, it is the cause for mental illness or cancer cells? I know last counter we learned about checkpoints in cell cycle and how cancer cells bypass and breakdown the checkpoints. Would the absence of Pop 129M have anything to do with the dysfunctional checkpoints since proteins are essential to cell and system function? Thank you for sharing.
Prion proteins are quite fascinating; I have researched them myself in the context of transmissible spongiform encephalopathy. I wonder if the protein you have researched here is the one responsible for TSE, or if it is just part of the same class of proteins. I am curious as to what properties of the prion protein allow it to act as a pathogen, despite the fact that it is less complex than even a virus.
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