Lab # 4: Blast by Hiu Nam (Tiger) Wong

• I searched for human muscle proteins. The reason behind is I find it interesting that many people nowadays, especially bodybuilders intake protein powders to boost muscle growth, therefore I would love to know more about the original muscle proteins found in human body.

• I selected the muscle protein from Homo sapiens, which has an accession number of CAC82104. Some intriguing facts about muscle proteins is that, since muscles accounts for almost 40% of our body weight, the number muscle proteins is the largest in all sorts of proteins, and they weigh about 5-6 kg.
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• This protein has an amino acid sequence of:
•      1 margplaarg lrlllpllpl lpllplpqva lgfadgscdp sdqcppqarw sslwhvglil
       61 lavlllllcg vtagcvrfcc lrkqaqaqph lpparqpcdv avipmdsdsp vhstvtsyss
       121 vqyplgmrlp lpfgeldlds mappayslyt pepppsydea vkmakpreeg palsqkpspl
       181 lgasglettp vpqesgpntq lppcspgap
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• Translating the first 10 amino acids are : margplaarg
• Methionine, Alanine, Arginine, Glycine, Proline, Leucine, Alanine, Alanine, Arginine, Glycine

• Citation:
• 
  
• REFERENCE   1
   AUTHORS: Ievolella,C., Zara,I., Frigimelica,E., Faulkner,G. and
             Lanfranchi,G.
   TITLE: Full length sequencing of some human and murine muscular
             transcripts (Telethon Italy project B41)
   JOURNAL: Unpublished
  REFERENCE   2  (residues 1 to 209)
   AUTHORS: Ievolella,C.
   TITLE: Direct Submission
   JOURNAL: Submitted (26-JUL-2000) Ievolella C., CRIBI Biotechnology
             Centre-Compl.Interdipartim. Vallisneri, Universita' di Padova, via
             Ugo Bassi 58/b, 35121, ITALY
• 
  

The nucleotide sequence of this protein is:

     1 gcttgaattc ggcgcgccag atatcacacg tgccaagggc tggctcaagc atggcccggg
     61 ggccgctggc cgcccgcgga ctgcggctgc tgctgccgct cctgccgctc ctgccgctcc
     121 tgccgctgcc gcaggtggcg ctgggcttcg cggacggcag ctgcgacccc tcggaccagt
     181 gcccgcccca ggcccgctgg agcagcctgt ggcacgtggg gctcatcctg ctggcggtcc
     241 tcctgcttct gctgtgtggt gtcacagctg gttgtgtccg gttctgctgc ctccggaagc
     301 aggcacaggc ccagccacat ctgccaccag cacggcagcc ctgcgacgtg gcagtcatcc
     361 ctatggacag tgacagccct gtacacagca ctgtgacctc ctacagctcc gtgcagtacc
     421 cactgggcat gcggttgccc ctgccctttg gggagctgga cctggactcc atggctcctc
     481 ctgcctacag cctgtacacc ccggagcctc caccctccta cgatgaagct gtcaagatgg
     541 ccaagcccag agaggaagga ccagcactct cccagaaacc cagccctctc cttggggcct
     601 cgggcctaga gaccactcca gtgccccagg agtcgggccc caatactcaa ctaccacctt
     661 gtagccctgg tgccccttga aggaggtagg agaacggacc agagcttgga gaactaatgc
     721 ttggagccaa gggccccagc ccaccccacc gtcccacaca ttgctgtggc cccaacctcg
     781 gtgccatgtt acaccggccc ctggcgtcac ccactaggca ggctgctgct ttcagcctca
     841 gcccctggcc cagccccagc aggccctcag cctggaagag gccccttggg cctaagcctc
     901 gggtgggagc tcagggccac ctgtgacgtc tgcatcttct tggagagaga ataaagtttg
     961 tatttaagtg g