Lab #4 BLAST by Morgan Howell

1). The protein I searched for was rhodopsin which is a protein found in the rods of the eye. Rods are a photoreceptor in the eye and they are what help you see in low amounts of light. Rhodopsin is the protein that, along with a molecule called 11-cis retinal, turns light into electrical signals that goes to the brain and the brain processes it as vision. I chose this protein because I think eyes are fascinating and I want to be an optometrist someday.

2). The specific protein I selected was rhodopsin [Homo sapiens].

3). The amino acid sequence is:

1 mngtegpnfy vpfsnatgvv rspfeypqyy laepwqfsml aaymfllivl gfpinfltly
      61 vtvqhkklrt plnyillnla vadlfmvlgg ftstlytslh gyfvfgptgc nlegffatlg
     121 geialwslvv laieryvvvc kpmsnfrfge nhaimgvaft wvmalacaap plagwsryip
     181 eglqcscgid yytlkpevnn esfviymfvv hftipmiiif fcygqlvftv keaaaqqqes
     241 attqkaekev trmviimvia flicwvpyas vafyifthqg snfgpifmti paffaksaai
     301 ynpviyimmn kqfrncmltt iccgknplgd deasatvskt etsqvapa

The first ten amino acids are:

Met-Asn-Gly-Thr-Glu-Gly-Pro-Asn-Phe-Tyr

Or

Methionine-Asparagine-Glycine-Threonine-Glutamic Acid-Glycine-Proline-Asparagine-Phenylalanine-Tyrosine

4). The first reference:

Xiong, S, et al. “Rhodopsin T17M Mutant Inhibits Complement C3 Secretion in Retinal Pigment Epithelium via ROS Induced Downregulation of TWIST1.” Journal of Cellular Biochemistry., U.S. National Library of Medicine, Dec. 2017, www.ncbi.nlm.nih.gov/pubmed/28569420.

5). The first 15 lines of the nucleotide sequence:

1 agagtcatcc agctggagcc ctgagtggct gagctcaggc cttcgcagca ttcttgggtg
      61 ggagcagcca cgggtcagcc acaagggcca cagccatgaa tggcacagaa ggccctaact
     121 tctacgtgcc cttctccaat gcgacgggtg tggtacgcag ccccttcgag tacccacagt
     181 actacctggc tgagccatgg cagttctcca tgctggccgc ctacatgttt ctgctgatcg
     241 tgctgggctt ccccatcaac ttcctcacgc tctacgtcac cgtccagcac aagaagctgc
     301 gcacgcctct caactacatc ctgctcaacc tagccgtggc tgacctcttc atggtcctag
     361 gtggcttcac cagcaccctc tacacctctc tgcatggata cttcgtcttc gggcccacag
     421 gatgcaattt ggagggcttc tttgccaccc tgggcggtga aattgccctg tggtccttgg
     481 tggtcctggc catcgagcgg tacgtggtgg tgtgtaagcc catgagcaac ttccgcttcg
     541 gggagaacca tgccatcatg ggcgttgcct tcacctgggt catggcgctg gcctgcgccg
     601 cacccccact cgccggctgg tccaggtaca tccccgaggg cctgcagtgc tcgtgtggaa
     661 tcgactacta cacgctcaag ccggaggtca acaacgagtc ttttgtcatc tacatgttcg
     721 tggtccactt caccatcccc atgattatca tctttttctg ctatgggcag ctcgtcttca
     781 ccgtcaagga ggccgctgcc cagcagcagg agtcagccac cacacagaag gcagagaagg
     841 aggtcacccg catggtcatc atcatggtca tcgctttcct gatctgctgg gtgccctacg