Lab #4: Blast by Na Nguyen

1.    What protein did you search for?

I searched for Raphanus sativus. It's common named of the red radishes or daikon.

2.    What specific protein did you select (be careful here – a search for insulin will also give you results for insulin receptors and many other insulin-related molecules)?

The specific protein I selected was threonine protein kinase.

3.    What is the amino acid sequence that codes for this protein? Cut and paste from the database and put these search results (or no more than the first 15 typed lines) in your lab report.  Translate the first 10 amino acids from this code into the amino acid names.

    1 menivacdcs fnnnmtchii elklkslsls gklppelakl qhlqkinlcr nylsgsipme       

  61 waslpnltym sfcanrlsgp lppglqnfks lqflgveanq fsgpipdelg nminltglel       

121 asnhftgslp sslarlvnld kfrisennfs gtipeyignw srlqrldlqa sgltgpfpda       

181 varlenltyl fisdmtgins fpnissqtik dlmlrnvsms gqipsyiwsk pdlrsldlsf       

241 nkltgevlgi nlapkftylt gnmlsgeiks gvylnsrsni dlsynnfswp sscqersnin       

301 tyrssslknt ltgllpcagp itckhykrsl hincggetvt vtnsigkity qadnsdkvka       

361 atnehfknwg isntgdfmdd ssdedtyiis tssrlpgdsp dlyktarrsa lslvyysfcl       

421 engeynlklh fmeiqfsdqe lysrlgrrif dvyvqgklfl rdfnirkean gtlksivkel       

481 kavnvtdhkl eiwlywagkg ttlipkrgny gplisaislc hsmeqhcgve ktkhhisyal       

541 ifgitgplia ifllalgfya qrkcrrdkkk rerdlraqgl qtvcftwrql qaatnnfdea       

601 nklgeggfgs vfkgeltdgt iiavkklssk scqgnrefvn eigmisglnh pnlvklygcc       

661 vekdqlllvy eymennslal alsgnsslkl dwaarkkict gtargldflh egaairmihr       

721 diktpnvlld adlnakisdf glarlheeeh thistkvagt igymapeyal mghltekadv       

781 ysfgvvamei vsgksnttqk gsddnaplik wamtlqqkgd imeivdpkle gefnsleaer       

841 mirvalvctn anpssrplms eavkmlegem eipqilsgpa vyghdlnfsk lmemqestsm       

901 sgyglspfnq gsatsnstae fss

                                menivacdcs

                Met – Glu – Asn – Ile – Val – Ala – Cys – Asp – Cys – Ser
Methionine – Glutamic acid/Glutamate – Asparagine – Isoleucine – Valine – Alanine – Cysteine – Aspartic acid/aspartate – Cysteine - Serine 

4.    Copy and paste the citation of the paper from which the data are drawn; you will need the authors, title, and journal. If there is more than one reference cited, use only the first.

 AUTHORS   Nie,S., Li,C., Xu,L., Wang,Y., Huang,D., Muleke,E.M., Sun,X.,
            Xie,Y. and Liu,L.
  TITLE     De novo transcriptome analysis in radish (Raphanus sativus L.) and
            identification of critical genes involved in bolting and flowering
  JOURNAL   BMC Genomics 17 (1), 389 (2016)

5.    What is the nucleotide sequence that codes for this protein? Cut and paste from the database and put these search results (or no more than the first 15 typed lines) in your post.

    1 aaaaaaaact ttcatttcac aattattatt ttatctcttc ttcttgttgc taaagattgt         

  61 gactaaccaa ccaagtccac ataatctata tcgatgatgt ggatcgtctt tttcttcatc       

121 atcattacaa gcttctttgc gactctaaca acatccactt caccagctct ccatccagat       

181 gaatgtactc aatgaaaatc ttggtcctta taatatcaaa ggtcagtatc cataactttt       

241 ttcgtatata atttctataa tttttgcagt gaaagcgctt ggggagatag cttctacact       

301 tggaatcaag agactgaacc taagtgatgg agatccttgt cttttaaaaa ctctaaaaat       

361 tggtcttgtt tctaattcgg attcggatat ggaaaatatc gttgcttgtg attgtagttt       

421 caacaacaac atgacatgtc atattataga actaaaactc aagtccttaa gtctttcagg       

481 gaaactcccg cctgagttgg ccaaacttca gcatctccag aagataaact tgtgccgaaa       

541 ctacctttct ggctcaatcc caatggagtg ggcttcattg ccaaacctca cttacatgtc       

601 gttctgcgcg aatcgtttgt ctgggccatt accacctggt ttacaaaact tcaagagtct       

661 acaattccta ggggttgaag ccaaccagtt ctctggtcct attcctgatg agcttggtaa       

721 catgatcaac ctaacaggat tggaacttgc gtccaatcat tttacaggaa gccttcctag       

781 ctctcttgct agactggtaa atcttgataa atttaggata agtgaaaata acttcagtgg       

841 caccatccca gaatatattg gcaactggtc tcggcttcaa agactagatt tacaagcaag