Meredith Swann's Lab 4: BLAST, Cordyceps militaris protein

What protein did you search for?

I chose to look for proteins created by any member of the Cordyceps family, and found a paper on a particular protein produced by Cordyceps militaris with possible antifungal and anticancerous properties here. For those unfamiliar, Cordyceps is a fungus known for parasitizing assorted insects, with two of the more well-known species growing in ants and caterpillars. For those of you who have heard of the video game The Last of Us, the zombie plague in that game is supposed to be a mutated strain of Cordyceps.

Very pretty-- when you can't see what it's growing out of! 😉

What specific protein did you select (be careful here – a search for insulin will also give you results for insulin receptors and many other insulin-related molecules)?

Serine cysteine tripsin, accession number ATY67355. The protein referred to as CMP in the paper I mentioned was not given a specific name and was only roughly described as a "trypsin-like serine protease," so it is likely not quite the same one.

What is the amino acid sequence that codes for this protein? Cut and paste from the database and put these search results (or no more than the first 15 typed lines) in your lab report.  Translate the first 10 amino acids from this code into the amino acid names.

    1 mipdisgtsh lelgaaated gkstkpnkpa pesplasdgl vtwsllgdqk qesikhtihg

  61 hdriraieas fdhedkrtpv dlgdfqdggk yrsivriesr wggertapwt igtgwlvred

121 llitaghnvf saahqcqash iqcqlgyrgg sfidesseqy rsalnvvttk swlrqhrdrd

181 rrdlaliqvd kpftgnlrlf kyiqtptskk egtiivvgyp gdehletfvg etslwmcear

241 qkanydldks anhmieynmd iyggqsgapi lfsdhtgtvv igthcysgtg gkvnsgslig

301 geygnkyedf ikrvelskre epyrlggfqp kfahldsyys efsrsggtnd fwaminkvke

361 iglysfgggq sltlgpvgwl mgtiaggmig llsatartes qkryesptwd linacacraq

421 laeaafqcvm rltpseerek vilkmrdywv dhlswlpfdt lsevhllvql spifndfgfr

481 lavsqwqkdi sanpevkplg qeflklprig qirfdkrgdg llwglfgvqs rfvhsvdqkn

541 vesaagwlag vvktaiqvaq pivseaaskd lrrivdamgm gdsgyipipd midqemgwiv

601 kravmadmal qvlenldfev laeltvmprg teresalani rtiiqqigpk pielakismm

661 klfdpiysrl leqkihpkav agsamakade apgephengn ktpahsptvv peiknv

The first ten amino acids are methionine, isoleucine, proline, aspartic acid, isoleucine, serine, glycine, threonine, serine, and histidine.

Copy and paste the citation of the paper from which the data are drawn; you will need the authors, title, and journal. If there is more than one reference cited, use only the first.

 AUTHORS   Kramer,G.J. and Nodwell,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Department of Biochemistry, University of
            Toronto, MaRS Centre West Tower, 606 University Avenue, Toronto, ON
            M5G1M1, Canada

What is the nucleotide sequence that codes for this protein? Cut and paste from the database and put these search results (or no more than the first 15 typed lines) in your post.

I could not find a nucleotide listed for this protein.