PDCD1 PROTEIN BLAST by Mackenzie Whittall

Cancer is unfortunately common in my family, and I have read previously about how cancer cells have the ability to avoid apoptosis, or "programmed cell death." Apoptosis emerged as an "irreversible process to efficiently eliminate dysfunctional cells," (Genentech USA, Inc [date unknown]) which could be extremely helpful in cancer treatments. I selected the protein PDCD-1 (Accesion: APS85787), or "Programmed cell death protein 1.” PDCD-1 promotes apoptosis in antigen-specific T-cells and reduces apoptosis in regulatory T-cells which ultimately inhibits the immune system and prevents autoimmune diseases. Unfortunately this can also keep the immune system from getting rid of cancer cells.

The PDCD-1 source for the following information is relating to Sarcophilus harrisii, also known as a Tasmanian devil, as the nucleotide for PDCD-1 in Homo sapiens was not available.

The amino acid sequence that codes for PDCD-1 is: 1 mvkteytlgf wplqlsrteg enatficnvs sklkapilnw yrekngsqpe klaaypkdtp 61 sshlqdryhi amkghkrfye mtilglrlnd sgryfcgiin ietptveesg rselnvteri 121 mvpttappvt tqaqsekfpw livipvvvga vllllllcwi lvvvefpgrg gaensgsnkd 181 amspkkaepc vasvstvvyg qldfqraeal kqggassdeq teyatiifpe eksafpgssp 241 hrk The first ten amino acids from this sequence (mvkteytlgf) are as follows: Methionine-Valine-Lysine-Threonine-Glutamic acid-Tyrosine-Threonine-Leucine-Glycine-Phenylalanine

The nucleotide sequence that codes for PDCD-1 in Sarcophilus harrisii is: 1 atggtgaaaa ccgaatatac ccttggcttt tggccactcc agctctcaag gacagaagga 61 gaaaacgcca cttttatctg caacgtctcc agcaagttaa aagctcccat cttaaactgg 121 taccgagaaa agaatggcag ccagccggag aagttagctg cctaccccaa agacacgccc 181 agctctcacc tgcaggacag gtaccacata gccatgaagg gccacaagcg gttctatgag 241 atgaccatct tggggctgcg gctgaacgac agtgggagat acttctgcgg aattatcaac 301 atcgaaacgc cgacagtgga agagagcggt cggtcagagc tcaatgtgac agaaagaatc 361 atggtcccga ccacagctcc tcctgtcacc acccaagcac aatccgaaaa gttcccgtgg 421 ctcattgtga tcccggtcgt cgtgggggct gtcctgctgc tgctgctgct ctgctggatt 481 ctggtcgtgg tagaattccc gggtcgagga ggggccgaga actcggggag caacaaggac 541 gcaatgtccc cgaagaaggc tgagccctgc gtggcgtcag tgtccaccgt ggtctacggt 601 cagctggact tccagagggc cgaggccctc aagcagggag gggccagctc ggacgagcag 661 acggaatacg ccaccatcat cttcccggag gagaagtctg cgtttcccgg cagctctccc 721 catcggaagt ga

Citation: REFERENCE 1 (residues 1 to 243) AUTHORS Flies,A.S., Lyons,A.B., Corcoran,L.M., Papenfuss,A.T., Murphy,J.M., Knowles,G.W., Woods,G.M. and Hayball,J.D. TITLE PD-L1 Is Not Constitutively Expressed on Tasmanian Devil Facial Tumor Cells but Is Strongly Upregulated in Response to IFN-gamma and Can Be Expressed in the Tumor Microenvironment JOURNAL Front Immunol 7, 581 (2016)

References

      “Evasion of Apoptosis: A Hallmark of Cancer.” Apoptosis Evasion in Cancer | BioOncology, Genentech USA, Inc.

      “PDCD1 [Sarcophilus harrisii] - Protein - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, 10 Jan. 2017.