Tien Van BLAST POST sec AA BIOL 212

Tien Van

Bio 212, sec AA

Winter 2018

Date: 1/17/2018

BLAST-AA

Blog Assignment #2

Lab 4 | BLAST  – Finding Protein Sequences

*Sequence assigned: sequence 7

I search for a neurotoxin protein which commonly finds in scorpions. Neurotoxins are disruptive to the brain because they inhibit neuron control over ion concentrations across the cell membrane which could lead to fatal harms.The specific protein I select is neurotoxin AaH II precursor. It has 370 nucleotides encoding precursors of toxins active on mammals and contain signal peptides of about 20 amino acid residues.

Figure 1: An image of a 3D structure of the protein.

Experimental method: X-ray diffraction.

Evolution: 2.3 Å

Figure 2: An image of Androctonus australis (Sahara scorpion), a typical animal which has the source of protein  neurotoxin AaH II

- nucleotide sequence

      1 aacaattcat ttttcctgct ttcctagaaa attctataaa agcttcaaaa tgaattactt
      61 ggtgatgatt agtttggcac ttctcttcgt gacaggtgta gagagtgtaa aagacggtta
     121 tattgtcgac gatgtaaact gcacatactt ttgtggtaga aatgcatact gcaacgagga
     181 atgtaccaag ttgaaaggtg agagtggtta ttgccaatgg gcaagtccat atggaaacgc
     241 ctgttattgc tataaattgc ccgatcatgt acgtactaaa ggaccaggaa gatgccatgg
     301 ccgataaatt ataagatgga atgtatccta agtatcaatg ttaaataaat ataatcaaaa
     361 aatt

-Amino acid sequence:

1 mnylvmisla llfvtgvesv kdgyivddvn ctyfcgrnay cneectklkg esgycqwasp
      61 ygnacycykl pdhvrtkgpg rchgr

 The First 10 Amino Acids name:

Met- Asn- Tyr- Leu- Val- Met- Ile- Ser- Leu- Ala

Source:

Authors: Bougis,P.E., Rochat,H. and Smith, L.A.

Title: Precursors of Androctonus australis scorpion neurotoxins.
           Structures of precursors, processing outcomes, and expression of a
           functional recombinant toxin II

Journal: J. Biol. Chem. 264 (32), 1989