RESEARCH UPDATE: IMPACT OF TEMPERATURE AND LIGHT EXPOSURE ON GROWTH RATE OF MEALWORMS

Research Update:
Impact of Temperature and Light Exposure on 
the Growth Rate of Mealworms

Group Members: Josselin Chambily, Travis Friesner, Bishoy Galil, Hiu Nam Wong

Research Question & Hypothesis

Do temperature and light have any effect on the growth rate of mealworms? We hypothesize that temperature and light will have an effect on the growth rate of mealworms. Testing this hypothesis we want our research to find out what factors contribute to their ideal habitat. Moreover, we predicted that mealworms that are nurtured in the dark at 72oF will grow the fastest compared to mealworms put under different conditions. Our main question for that project is how temperature is important for the rate of growth of mealworms.

Discussion

o    Describe what you have found so far in terms of trends in your data.
We have found that temperature and light do affect the growth of our mealworms. Even if the effect of temperature is more obvious than the presence of lights, we can notice than the population exposed to higher temperature and constant light grew larger while the population conserved at low temperature in the dark shows the least amount of growth for the first 3 days. After this first period of time, their growth didn’t change as we expected: So far, we have found that all the populations grew bigger and heavier for the first 3 days, then all started to decrease in mass and size. A possible explanation could be the fact that mealworms are preparing for pupation, which is the next stage of their life cycle (we even observed few small and light pupae in some populations). To sum up, even if our mealworms decreased in size and mass around the end of our experiment in an unexpected way, we can clearly observe a trend where our mealworms grew more when they were exposed to light and conserved in higher temperatures.
o    What challenges or surprises did your group encounter?
First, it is hard to control the population growing conditions. We figured out that the room temperature is greatly affected by the weather. We aimed for a room temperature that is about 25oC hoping for a more obvious growth, yet the room temperature was about 20oC and fluctuates depending on the weather. Also, since part of our mealworm populations will be put under lights 24 hours, the heat from the light increases the temperature as well. Next, we have to figure out how to provide light source for enlighten populations that are put in incubator or fridge (which are dark when you close the door), ultimately, we had to buy LED lights from dollar stores and put them next to the mealworm populations, at the same time risking that the lights will die out during weekend. One surprise and challenge we faced after the first weekend, is that the mealworm populations put in 37oC molded badly, and all the mealworms were dead. We quickly concluded that 37oC was too high for mealworms, and changed the “higher temperature” population to a 27oC incubator.
o    What advice or solutions do you have for other groups?
 All groups need to be prepared for their experiments, as we can see that the experiments many times don’t go with our predictions. Thus, every group should make sure to have enough time is anything doesn’t go with their predictions. Moreover, every group should do their best trying to use precise instruments for measurements. Also, the measurements need to be taken more than one time to reduce the effect of error on the results. Let’s say that we are doing science, so it’s possible if we got imprecise results. So, every group has to think about limitations and try to organize the experiment considering those limitations.    
o    What will you discuss in your error analysis section of your discussion?
We faced many challenges during our experiment. To begin, keeping the temperature constant for the populations throughout the whole experiment was really hard; extended periods outside of the incubator during growth measurements may be an error that affected our results. The 24 hours lights had an impact on surrounding temperature of the populations as we noticed that the temperature under the lights was actually 28oC rather than a desired 25oC. Because of this additional heat from the lights, there was a temperature difference between the light and dark populations. Moreover, high humidity promoted mold growth that caused mealworms to die in the high temperature populations. Also, the nutrients we used, like potatoes and carrots, were not of the same mass which could affect the growth of our mealworms. The unequal amount of food supply might favor mealworms’ growth in populations that had more food, which leads to inaccurate results. Our experiment might have faced many human errors as measuring the length of the worms were very hard to be precise.

What question(s) do you have for other groups?

How did your group make sure your variables remains constant, or at the desired level/measurements?

Do the data your group obtained support your hypothesis?

What are some surprising findings?

Comments

  1. Hi guys! your experiment looks so fun! I liked how thorough your guys experiment, and this post is. After reading this, it helped me better grasp the life of insects in the beginning of their life. i was curious what instruments would give better calculations than the ones you used. I can only think of the ruler and placing the mealworms next to it.

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  2. I was very interested in your groups analysis of the results because I noticed the over growth of mold during lab. Some of the surprising findings in my group's experiment was the changing of pH of buffer, plus most of our organism (Daphnia magna) died in the same day and we had to come up with plan B, but still most of them still died. As a result we had to revise our hypothesis.

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  3. I was curious to see how you experiment turned out. It was interesting to see that you guys had to change the procedure a little. I think most of the groups had to check how the first trial would go then modify it if necessary. I know that my group had to do that. I was wondering how you guys were measuring the size of the worms, but I guess I'll find out during your presentation.

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  4. My group also experienced a large die-off with our organisms, although in our case we lost almost all of our Daphnia. Were you able to replace the lost mealworms or did you just make do with a reduced sample size? Also, did you observe mold growth in any of your experimental conditions other than 37°C? I would be interested to know how the mealworms in that population would have fared without the mold present.

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  5. Hi guys, luckily non of our pill bugs died during our experiment however our results didn't always fully support our hypothesis. We initially thought that the pill bugs would have preference towards the Karo syrup soaked leaves because it would be closer and more similar to components found in their natural environment. However in the final trial of our experiment we used potato bugs instead of pill bugs and the potato bugs were wild caught. So it would have been better for us to start with wild caught potato bugs because their preference is probably more accurate to the true diet preferences of wild pill bugs. The pill bugs seemed to have a preference mainly of the Karo syrup but when we used the wild caught potato bugs they preferred our control environment much more. So we tried to keep a lot of things constant but their is more things we would have done differently to keep things more constant and our results more accurate.

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  6. Hi guys, luckily non of our pill bugs died during our experiment however our results didn't always fully support our hypothesis. We initially thought that the pill bugs would have preference towards the Karo syrup soaked leaves because it would be closer and more similar to components found in their natural environment. However in the final trial of our experiment we used potato bugs instead of pill bugs and the potato bugs were wild caught. So it would have been better for us to start with wild caught potato bugs because their preference is probably more accurate to the true diet preferences of wild pill bugs. The pill bugs seemed to have a preference mainly of the Karo syrup but when we used the wild caught potato bugs they preferred our control environment much more. So we tried to keep a lot of things constant but their is more things we would have done differently to keep things more constant and our results more accurate.

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  7. Hey guys! great post. As depressing as it sounds, death is data. It is a good thing you guys continued to move forward with the project and do some revising! it's always best to have a backup plan. I love the fact that your group's project was different from others in the room. I'm very excited to see how your result look like in the presentation.

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  8. This was a really interesting idea to test out. Bummer that not all the worms survived. I think in the future to make it easier with variables is to limit how many you are changing. Like doing trials with just temperature change and then doing separate ones with only lighting changes. If you did choose to only doing lighting change then it could be interesting to see if different colors of light might play an effect., but it could also effect temperature if you don't watch it. I have heard of things you can buy that will watch the heat and turn the light off (or getting a heat mat for the temp. change) when the deserved temperature is reached and turned back on when it cools down. If you could get something like that and keep them all in same lighting then I think it would be more ideal in this case. Hopefully that helps if you are thinking of trying it again in the future!

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  9. It's refreshing to see a different organism in the cluster of these research projects. I honestly wouldn't even think that temperature affects mealworms. I wonder if the dirt that they live in and burrow themselves into keeps them at a steady weight (and they do that on purpose) for the next stage since you guys found out that the light radiation makes them a little chunkier. Maybe for the next research experiment you guys can do the feeding types on mealworms. Anyways, great post!

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