Blog Post #2 BLAST Protein Sequence - Alanna Barnett

1. The protein I chose to search for this assignment was a prion protein; ideally a human prion protein. Given that these proteins have the potential for catastrophic damage to the brain when folded improperly, I thought they would make an interesting choice.
   
2. The particular prion protein (PrP) that I used was the Prion Protein [Homo Sapiens], accession AAH22532.
   
   
              Image from NCBI database
3. Amino acid sequence:
   
       1 manlgcwmlv lfvatwsdlg lckkrpkpgg wntggsrypg qgspggnryp pqggggwgqp
     61 hgggwgqphg ggwgqphggg wgqphgggwg qgggthsqwn kpskpktnmk hmagaaaaga
   121 vvgglggyvl gsamsrpiih fgsdyedryy renmhrypnq vyyrpmdeys nqnnfvhdcv
   181 nitikqhtvt tttkgenfte tdvkmmervv eqmcitqyer esqayykrgs smvlfssppv
   241 illisflifl ivg
   
   First 10 AA, translated:
   
   Methionine, Alanine, Asparagine, Leucine, Glycine, Cysteine, Tryptophan, Methionine, Leucine, Valine
   
4. Citation:
   
   Mammalian Gene Collection (MGC) Program Team. (2002). Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America, 99(26), 16899–16903.
   http://doi.org/10.1073/pnas.242603899
   
5. Nucleotide sequence:

          1 agtcgctgac agccgcggcg ccgcgagctt ctcctctcct cacgaccgag agcagtcatt
        61 atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag tgacctgggc
      121 ctctgcaaga agcgcccgaa gcctggagga tggaacactg ggggcagccg atacccgggg
      181 cagggcagcc ctggaggcaa ccgctaccca cctcagggcg gtggtggctg ggggcagcct
      241 catggtggtg gctgggggca gcctcatggt ggtggctggg ggcagcccca tggtggtggc
      301 tggggacagc ctcatggtgg tggctggggt caaggaggtg gcacccacag tcagtggaac
      361 aagccgagta agccaaaaac caacatgaag cacatggctg gtgctgcagc agctggggca
      421 gtggtggggg gccttggcgg ctacgtgctg ggaagtgcca tgagcaggcc catcatacat
      481 ttcggcagtg actatgagga ccgttactat cgtgaaaaca tgcaccgtta ccccaaccaa
      541 gtgtactaca ggcccatgga tgagtacagc aaccagaaca actttgtgca cgactgcgtc
      601 aatatcacaa tcaagcagca cacggtcacc acaaccacca agggggagaa cttcaccgag
      661 accgacgtta agatgatgga gcgcgtggtt gagcagatgt gtatcaccca gtacgagagg
      721 gaatctcagg cctattacaa gagaggatcg agcatggtcc tcttctcctc tccacctgtg
      781 atcctcctga tctctttcct catcttcctg atagtgggat gaggaaggtc ttcctgtttt
      841 caccatcttt ctaatctttt tccagcttga gggaggcggt atccacctgc agccctttta